Coding

Part:BBa_K3257036

Designed by: Yi Qiao   Group: iGEM19_Fudan-TSI   (2019-09-05)


Chloramphenicol Resistance Gene Y92X (ChlR_Y92X)

This part is a mutant of a gene encoding ChlR, a resistance protein of chloramphenicol, and it is nonsense mutated at its 92nd amino acid residual. We used this mutated ChlR as one of the target genes of our directed evolution process. After expression in BL21(DE3), we counted the number of colonies grown on a medium plate containing chloramphenicol to show its inability of being resistant to chloramphenicol.

Numbers of different types of colonies grown on plates with different antibiotics (Data of this part shown in red)

No ChlR WT ChlR ChlR_T33X ChlR_Y92X ChlR_E97X ChlR_S121X ChlR_L158X ChlR_K182X ChlR_Q190X
Amp
19
192
291
128
236
138
255
175
276
Amp/Chl
0
30
0
0
0
0
0
0
0


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None